Engineers will re-align the crossing (Cornton 2) from 11pm on Friday 9 February until 5.30am on Monday 12 February, with no access for pedestrians during that time.
New gates will be installed in a different position than currently is the case, with more secure fencing being put in place and both miniature stop lights being moved to the left-hand side of each gate.
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Network Rail is working closely with local stakeholders and Stirling Council to minimise the duration of the closure.
Christopher Sharkey, Senior Programme Manager, Network Rail, said: "The planned work at Cornton pedestrian level crossing is essential to maintaining a safe and reliable railway in the area.
"While we appreciate that the short-term impact of this work is inconvenient, the long-term benefit is that it will minimise overall disruption for both passengers and pedestrians while helping us to deliver a safe, reliable and resilient railway.
"Our thanks go to the community for their patience and understanding while we complete this vital work."
